Genomic DNA Extraction: Used to resuspend and lyse cells/tissues while EDTA protects nucleic acids from nucleases.

Plasmid Preparation: Employed for resuspending Escherichia coli pellets and protecting plasmid DNA during alkaline lysis workflows.

DNA Storage: Serves as a storage buffer for purified DNA, maintaining stable pH and ionic strength.

Nuclease Inhibition: EDTA chelates divalent cations to inhibit DNases/RNases during sample handling.

Sample Dilution: Provides consistent conditions for diluting nucleic acids, enzymes, and standards.

Proteinase K/SDS Digestions: Functions as the base buffer for proteolytic lysis during DNA isolation.

Phage/Viral Particle Handling: Used to resuspend, stabilize, and store bacteriophages and viral preparations.

Nuclei Isolation: Applied in isolating intact nuclei and preparing chromatin by maintaining osmotic balance and pH.