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T0310

10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$92.00

Fill Volume

1000 mL

T0310

10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

10X Tris-Buffered Saline (TBS) with 0.5% Tween-20 is a concentrated wash and dilution buffer for immunodetection workflows. The Tris-based saline maintains physiological ionic strength and buffering capacity, and the nonionic surfactant aids in gentle membrane and surface washing.

Suitable for preparing working-strength buffer for Western blotting, ELISA, immunohistochemistry, and general washing steps, including antibody dilutions and membrane rinses. Dilute 1:10 with distilled water to obtain 1X TBS-T for routine use.

  • 247mM Tris
  • 27mM Potassium Chloride
  • 1.37M Sodium Chloride
  • 0.5% Tween 20
  • Western Blotting Wash Buffer: Reduces nonspecific antibody binding during membrane wash steps for clear signal.

  • Western Blot Antibody Diluent: Maintains antibody stability and minimizes background during primary/secondary incubations.

  • ELISA Plate Wash: Efficiently removes unbound reagents from microplates to improve signal-to-noise.

  • ELISA Antibody Diluent: Supports consistent antibody performance with reduced nonspecific interactions.

  • Immunohistochemistry (IHC): Used for tissue section washing and blocking steps to limit background staining.

  • Immunocytochemistry/Immunofluorescence (ICC/IF): Wash and blocking buffer for cells to preserve signal specificity.

  • Dot/Slot Blot Assays: Wash buffer to clear unbound probes and antibodies from membranes.

  • General Blocking/Washing: For protein- and membrane-based assays where mild detergent reduces nonspecific binding.

  • Flow Cytometry Staining Wash: Gentle washing of labeled cells to decrease background fluorescence.

  • Bead-Based Immunoassays: Wash and dilution buffer to limit nonspecific adsorption on magnetic or agarose beads.

  • pH: 7.40 ± 0.04
Storage Temperature
Room Temperature
Shelf Life
1095 days
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