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T0275

5X TBE DNA Sequencing Grade Solution. 4L, Non-Sterile.

Availability

Ships in 6 - 14 Days

Container Size

4L

Item Count

1 Bottle(s)

Price per Item

$135.00

Fill Volume

4000 mL

T0275

5X TBE DNA Sequencing Grade Solution. 4L, Non-Sterile.

Quantity

Minimum is set to 4 for this product*

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Description
Formulation
Common Applications
Storage

5X TBE DNA Sequencing Grade Solution, Non-Sterile is a concentrated Tris-borate-EDTA buffer for the preparation and electrophoretic separation of nucleic acids. It is intended for dilution to a working concentration for casting agarose or polyacrylamide gels and for use as a running buffer.

This solution is suited for DNA sequencing workflows and general molecular biology protocols that require defined buffering capacity and chelation of divalent cations. Supplied non-sterile; sterilization may be performed by the user if needed.

  • 445mM Tris
  • 445mM Boric Acid
  • 10mM Ethylenediaminetetraacetic acid (EDTA)
  • Agarose Gel Electrophoresis (DNA): Used as a running and gel-casting buffer for high-resolution separation of DNA fragments.

  • Polyacrylamide Gel Electrophoresis (DNA Sequencing): Provides a stable ionic environment for denaturing urea-PAGE in DNA sequencing workflows.

  • Pulsed-Field Gel Electrophoresis (PFGE): Employed (typically diluted to 0.5X–1X) for resolving large DNA molecules with consistent buffering capacity.

  • Capillary Electrophoresis (CE) of DNA: Serves as a conductive buffer for nucleic acid analysis in CE systems.

  • PCR Amplicon Analysis: Enables post-PCR verification of fragment size and integrity during gel runs.

  • DNA Fragment Isolation/Electroelution: Maintains DNA integrity and pH during recovery from agarose or polyacrylamide gels.

  • RNA Denaturing PAGE: Supports high-resolution separation of RNA in urea-PAGE for analytical workflows.

  • Plasmid Prep QC: Used to assess plasmid digests and preparations from Escherichia coli by agarose gel electrophoresis.

Storage Temperature
Room Temperature
Shelf Life
365 days
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