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T0258

DNA Suspension Buffer, RNAse DNAse Tested (10mM Tris, 0.1mM EDTA), pH 8.0. 500mL, Sterile

Availability

Ships in 6 - 14 Days

Container Size

650mL

Item Count

1 Bottle(s)

Price per Item

$93.00

Fill Volume

500 mL

T0258

DNA Suspension Buffer, RNAse DNAse Tested (10mM Tris, 0.1mM EDTA), pH 8.0. 500mL, Sterile

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

DNA Suspension Buffer, RNase DNase Tested, pH 8.0 is a buffered aqueous solution for resuspension, storage, and dilution of DNA, including plasmid DNA, PCR products, and genomic DNA.

The pH 8.0 buffering system helps maintain solution pH during handling, and the chelating agent limits free divalent cations that support nuclease activity. Each lot is tested for RNase and DNase to verify absence at detectable levels.

  • 10mM Tris-HCl
  • 0.1mM Ethylenediaminetetraacetic acid (EDTA)
  • DNA Resuspension and Storage: Resuspends and preserves genomic, plasmid, and PCR products, maintaining pH 8.0 with low EDTA to protect against nucleases.

  • Plasmid DNA from Escherichia coli: Hydrates and stores plasmids from bacterial minipreps/maxipreps for downstream applications.

  • NGS Library Handling: Rehydrates, dilutes, and stores next-generation sequencing libraries prior to pooling and loading.

  • Oligonucleotide Reconstitution: Dissolves primers and probes for PCR, qPCR, and sequencing workflows.

  • PCR/qPCR/ddPCR Template Dilution: Dilutes DNA templates and standards to minimize adsorption and maintain integrity.

  • Sanger Sequencing Preparation: Dilutes purified PCR products or plasmids for capillary sequencing.

  • Spin-Column DNA Elution: Serves as a low-salt elution buffer for silica-based DNA purification.

  • Standard Curve Preparation: Prepares DNA calibrators for qPCR and fluorometric quantification assays.

  • Sample Storage and Transport: Provides nuclease-tested conditions for short- to medium-term DNA storage and shipment.

  • Cloning Workflow Support: Maintains DNA stability for downstream cloning and assembly steps.

  • pH: 8.00 ± 0.10
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
730 days
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