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T0238

TE Buffer (10mM Tris, 1mM EDTA), pH 8.0, 4L, RNase/DNase/PCR Tested, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

4L

Item Count

1 Bottle(s)

Price per Item

$407.00

Fill Volume

4000 mL

T0238

TE Buffer (10mM Tris, 1mM EDTA), pH 8.0, 4L, RNase/DNase/PCR Tested, Sterile.

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

TE Buffer, pH 8.0, is a buffering solution used for the preparation, storage, and dilution of DNA and RNA. It maintains stable pH and chelates divalent cations that can promote nuclease activity, and each lot is tested for RNase, DNase, and PCR.

  • 10mM Tris-HCl
  • 1mM Ethylenediaminetetraacetic acid (EDTA)
  • Nucleic Acid Storage: Long-term storage of plasmid, genomic DNA, and RNA at pH 8.0; EDTA helps protect against nuclease degradation.

  • DNA/RNA Resuspension and Dilution: Rehydrates and dilutes purified nucleic acids, primers, and oligonucleotides.

  • Elution Buffer for Purification: Used to elute DNA from spin columns or magnetic beads to enhance stability versus water.

  • Sequencing and qPCR Prep: Dilutes and stores templates and primers for Sanger/NGS workflows and qPCR.

  • Cloning and Transformation: Handles and stores plasmid DNA for downstream restriction/ligation workflows and transformation into E. coli.

  • Reaction Quenching: Stops restriction enzyme or nuclease reactions by chelating divalent cations required for activity.

  • Probe and Library Storage: Maintains DNA/RNA probes and NGS libraries for consistent performance.

  • RNA Applications: RNase-free buffer for handling, diluting, and storing RNA, siRNA, and mRNA standards.

  • Gel Extraction Cleanup: Elutes and stabilizes DNA recovered from agarose gels.

  • pH: 8.00 ± 0.04
  • Conductivity: 700-900µS/cm
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
1095 days
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