Agarose Gel Electrophoresis of DNA/RNA: Used as 1X running and gel-casting buffer for sharp band resolution and stable pH.

Polyacrylamide Gel Electrophoresis for DNA Sequencing: Employed in denaturing or native PAGE (e.g., urea-PAGE) for high-resolution separation of nucleic acids.

Southern and Northern Blotting: Serves as electrophoresis and transfer buffer for nucleic acid blot workflows.

Oligonucleotide and ssDNA Analysis: Provides buffering and chelation for high-resolution PAGE of short nucleic acids.

Electrophoretic Mobility Shift Assays (EMSA): Used as running buffer in nucleic acid–protein binding assays.

DNA Fragment Recovery from Gels: Supports excision and electroelution/electrotransfer of DNA fragments.

General Nuclease Inhibition During Nucleic Acid Handling: EDTA chelates divalent cations to protect DNA/RNA from nuclease activity.