SDS-PAGE Sample Preparation: Denatures proteins and imparts uniform negative charge for size-based separation.

Protein Solubilization: Solubilizes aggregate and membrane proteins for downstream analysis.

Cell Lysis: Disrupts cell membranes of E. coli, Staphylococcus aureus, and mammalian cells to release proteins and nucleic acids.

Nucleic Acid Purification: Used with proteinase K to remove proteins and inactivate nucleases during DNA/RNA isolation.

Western Blotting: Components in sample buffers and some stripping buffers to denature proteins and elute antibodies.

Membrane Protein Extraction: Disrupts lipid bilayers to extract and analyze membrane-associated proteins.

Inclusion Body Solubilization: Solubilizes protein inclusion bodies prior to refolding workflows.

Virus and Microbial Inactivation: Disrupts enveloped viruses and denatures microbial proteins for safe sample handling.

Buffer and Reagent Preparation: Stock for preparing Tris-Glycine-SDS electrophoresis and related transfer buffers.

Labware Decontamination: Detergent-based removal of proteins, lipids, and residual biomolecules from equipment and surfaces.