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S3379

20% Sarkosyl Solution. 200mL, Sterile

Availability

Ships in 6 - 14 Days

Container Size

250mL

Item Count

1 Bottle(s)

Price per Item

$118.00

Fill Volume

200 mL

S3379

20% Sarkosyl Solution. 200mL, Sterile

Quantity

Minimum is set to 4 for this product*

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Description
Formulation
Common Applications
Storage

20% Sarkosyl Solution is an anionic detergent stock used for cell lysis, membrane solubilization, and nucleic acid purification workflows. It is typically diluted to 0.1–2% in buffer for plasmid preparations, genomic DNA isolation, and RNA extraction from bacterial and mammalian cells. The reagent disrupts Gram-negative bacterial outer membranes, including E. coli, and is compatible with common lysis buffer components such as EDTA and chaotropic salts. Supplied as an aqueous 20% w/v solution for laboratory use.

  • 20% Sodium Lauroyl Sarcosine Solution
  • Membrane Protein Solubilization: Efficiently solubilizes and stabilizes membrane proteins for purification and downstream analysis.

  • Cell Lysis for Nucleic Acid Extraction: Disrupts cells and envelopes to extract high-quality DNA/RNA from bacteria (e.g., Escherichia coli, Staphylococcus aureus, Bacillus subtilis), yeast, and mammalian cells.

  • Viral Inactivation and NA Extraction: Denatures viral envelopes and proteins to inactivate virus and release nucleic acids for qPCR/NGS workflows.

  • Ribosome Profiling and Polysome Analysis: Used to disaggregate polysomes and prepare ribosome footprints for Ribo-seq and sucrose gradient fractionation.

  • Chromatin/Nuclear Lysis: Facilitates stringent nuclear and chromatin lysis for applications such as ChIP and nuclear run-on (GRO/PRO-seq).

  • Inclusion Body Solubilization: Solubilizes aggregated or misfolded proteins from inclusion bodies prior to refolding protocols.

  • Endotoxin/LPS Reduction: Helps remove or reduce lipopolysaccharide contamination during plasmid and protein preparations.

  • Metagenomic DNA Extraction: Enhances lysis of tough environmental and clinical samples (e.g., soil, fecal) for unbiased microbiome sequencing.

  • Protein Sample Preparation: Maintains solubility of hydrophobic proteins during denaturation and electrophoresis workflows.

  • Biofilm and Tough Cell Wall Disruption: Assists in breaking down biofilms and robust cell walls to improve recovery of intracellular targets.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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