Bacteriophage Storage and Stabilization: Maintains phage integrity during short- and long-term storage, leveraging Tris-HCl buffer, Mg2+ for capsid stability, and gelatin to reduce adsorption.

Phage Dilution and Transport: Used as an isotonic diluent for accurate serial dilutions and safe transport of phage samples.

Plaque Assays and Titer Determination: Employed as a diluent and recovery buffer for phage plating on Escherichia coli lawns.

Elution from Agar Plugs/Plates: Facilitates efficient recovery of phage from top agar plugs, plaques, and environmental samples.

Resuspension and Dialysis of Phage Particles: Provides a gentle, stabilizing matrix for concentrating, washing, and dialyzing phage.

Propagation of Lambda/T4/T7 Phages: Supports preparation of high-titer stocks during amplification in Escherichia coli.

Phage DNA Preparation Workflows: Used for capsid cleanup, nuclease treatments, and resuspension prior to DNA extraction.

Host Range and Infection Studies: Serves as a standardized medium for recovering phage from infected cultures of Escherichia coli, Salmonella, and related hosts.