sacB-Based Counterselection for Allelic Exchange: Selects for loss of sacB-containing integrations in Gram-negative hosts (e.g., Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae, Salmonella enterica) using 6.0% sucrose lethality.

pBAD-sacB Induction: 0.2% L-(+)-Arabinose induces sacB under pBAD control to enable negative selection during recombineering workflows.

CRISPR-Assisted Editing Negative Selection: Enriches for correctly edited clones by counterselecting sacB-marked intermediates after CRISPR/recombineering.

Plasmid Curing of sacB-Tagged Vectors: Eliminates sacB-bearing suicide or helper plasmids following integration/excision cycles.

Screening Post-λ Red or Flp-FRT Steps: Identifies double-crossover recombinants after marker exchange or cassette excision.

Transposon Mutagenesis Workflows: Resolves insertions when transposon delivery or helper constructs carry sacB for counterselection.

Isolation of Sucrose-Resistant Variants: Screens for sacB loss-of-function or recombination events that confer growth on 6.0% sucrose.