Buffer and Media Preparation: Used to adjust ionic strength when preparing PBS, TBS, and related saline buffers from a 5M Sodium Chloride stock.

Nucleic Acid Hybridization and Blotting: Formulates SSC/SSPE buffers to control stringency for Southern/Northern blot workflows.

Ion Exchange Chromatography: Creates salt gradients and elution buffers to modulate protein binding and release.

Saline Solutions for Cell Handling: Diluted to make 0.9% saline for washing, resuspension, and serial dilutions of Escherichia coli and Staphylococcus aureus.

Phage Precipitation: Combined with PEG for purification of filamentous phage from Escherichia coli cultures.

Protein Solubility and Stability Screening: Titrates ionic strength to reduce nonspecific aggregation and optimize buffer conditions.

Immunoassay Wash Buffers: Prepares PBS/TBS-based wash buffers (e.g., PBST/TBST) for ELISA and Western blot workflows.

Culture Media Formulation: Adjusts NaCl levels in microbiological media (e.g., LB) for growth of Escherichia coli and Bacillus subtilis.

DNA/RNA Precipitation Workflows: Provides salt for select nucleic acid precipitation or cleanup protocols when compatible.

General Laboratory Reagent: Rapidly increases ionic strength in lysis, extraction, and storage buffers requiring precise NaCl concentrations.