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P4138

2.5M Sodium Chloride, 20% PEG Solution. 1000mL, Non-Sterile.

Availability

Ships in 1 - 2 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$119.00

Fill Volume

1000 mL

P4138

2.5M Sodium Chloride, 20% PEG Solution. 1000mL, Non-Sterile.

Quantity

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Description
Formulation
Common Applications
Storage

This saline–polyethylene glycol solution is supplied non-sterile and is prepared for use in laboratory workflows that employ PEG/NaCl-mediated precipitation. It is suitable for procedures such as the concentration of filamentous bacteriophages (e.g., M13, f1) and precipitation steps in nucleic acid handling protocols.

For applications requiring sterility, sterilize by filtration or other validated methods prior to use. Mix thoroughly before dispensing to ensure homogeneity, and store according to laboratory best practices to maintain integrity.

  • 2.5M Sodium Chloride
  • 20% Polyethylene Glycol 8000
  • Bacteriophage Precipitation and Concentration: Enriches filamentous and lytic phages (e.g., M13, λ, T7) from E. coli culture supernatants for downstream titering and sequencing.

  • Viral Particle Concentration: Concentrates enveloped and non-enveloped viruses from cell culture media to improve transduction and detection sensitivity.

  • Plasmid DNA Cleanup and Concentration: Precipitates plasmid DNA from bacterial lysates to increase yield and remove soluble contaminants.

  • Small RNA and Oligonucleotide Precipitation: Enhances recovery of short RNAs and oligos from dilute solutions or enzymatic reactions.

  • Protein Crystallization: Serves as a precipitant/crowding component in crystallization screens to promote crystal formation.

  • Macromolecular Crowding in Assays: Provides crowding and ionic strength control for protein–nucleic acid interactions and assembly reactions.

  • Phage Display Workflows: Concentrates phage particles and aids in cleanup between panning rounds to improve library handling.

  • Environmental Phage Concentration: Aggregates and concentrates bacteriophages from wastewater or environmental samples for metagenomic analysis.

Storage Temperature
Room Temperature
Shelf Life
730 days
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