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P4137

2.5M Sodium Chloride, 20% PEG Solution. 500mL, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

650mL

Item Count

1 Bottle(s)

Price per Item

$106.00

Fill Volume

500 mL

COA
COA

P4137

2.5M Sodium Chloride, 20% PEG Solution. 500mL, Sterile.

Quantity

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Description
Formulation
Common Applications
Storage

2.5 M Sodium Chloride, 20% PEG Solution is an aqueous mixture of sodium chloride and polyethylene glycol suitable for PEG/NaCl precipitation workflows. It is commonly used for concentrating filamentous bacteriophages from E. coli cultures, for viral particle precipitation, and for PEG-mediated nucleic acid precipitation procedures.

Intended for laboratory process steps where a combined salt and polyethylene glycol solution is specified by protocol, including phage precipitation and sample concentration applications.

  • 2.5M Sodium Chloride
  • 20% Polyethylene Glycol 8000

  • Bacteriophage Precipitation and Concentration: PEG/NaCl precipitation of M13 and λ phage from Escherichia coli culture supernatants for downstream DNA prep or titering.

  • Viral Particle Concentration: Concentrates viral vectors (e.g., AAV, lentivirus) from cell culture supernatants using PEG/NaCl precipitation.

  • DNA Cleanup/Precipitation: Selective precipitation to concentrate PCR amplicons or plasmid DNA and remove small contaminants.

  • RNA Precipitation: Enriches high–molecular-weight RNA species via PEG/NaCl-mediated precipitation.

  • Protein Crystallization Screens: Serves as a combined precipitant (PEG 8000) and salt (NaCl) condition in crystallography trials.

  • Extracellular Vesicle Isolation: PEG-based precipitation of exosomes and microvesicles from biofluids or conditioned media.

  • Macromolecular Crowding Studies: Provides high ionic strength with a crowding agent for phase separation and assembly assays.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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