Periplasmic Protein Extraction (Osmotic Shock): Releases periplasmic proteins from Gram-negative cells (e.g., Escherichia coli, Pseudomonas aeruginosa) using Tris-EDTA with 20% sucrose for osmoprotection.

Spheroplast Formation: Generates and stabilizes spheroplasts from Gram-negative bacteria via EDTA-mediated outer membrane permeabilization in a high-sucrose environment.

Periplasmic Enzyme Assays: Provides a buffered, chelator-containing medium to assay enzymes like alkaline phosphatase or beta-lactamase from periplasmic fractions.

Outer Membrane/Periplasmic Fractionation: Serves as an isotonic buffer for differential extraction and downstream proteomics of periplasm and outer membrane.

Phage Display Screening (Periplasmic Expression): Facilitates gentle recovery of scFv/Fab fragments expressed in the periplasm of Escherichia coli for binding assays.

Osmoprotective Wash Prior to Lysis: Stabilizes Gram-negative cells before mechanical lysis to minimize premature periplasmic leakage.

Membrane Vesicle Preparation: Acts as a sucrose-based cushion or resuspension medium for outer membrane vesicles from organisms like Escherichia coli and Salmonella enterica.

Plasmid Screening of Periplasmic Fusions: Recovery of periplasmically targeted recombinant proteins for rapid screening without full cell lysis.