Auxotrophic Selection (ADE+): Selects Pichia colonies that complement adenine auxotrophy on adenine-dropout plates.

Transformant Screening: Screens Pichia transformants carrying ADE-based selectable markers after integration or plasmid uptake.

Colony Isolation and CFU Enumeration: Enables clonal isolation and colony counting under adenine-selective pressure.

Plasmid Maintenance: Maintains ADE-marked expression plasmids in Pichia using adenine dropout selection.

Color-Based Phenotyping (ade2): Supports red/white colony phenotype assessment in ade2 backgrounds on adenine-limited medium.

Library and Mutant Screening: Selects ADE-marked mutant or genomic libraries grown on Pichia selective agar.

Integrant Stability Testing: Monitors stability of ADE-selected genomic integrants over passages on selective plates.

Metabolic Pathway Studies: Assesses purine biosynthesis and adenine salvage pathway phenotypes in Pichia.