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P0220

1X PBS-TBN Buffer with 0.05% Tween, 1% BSA, 0.1% Sodium Azide. 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$131.00

Fill Volume

1000 mL

P0220

1X PBS-TBN Buffer with 0.05% Tween, 1% BSA, 0.1% Sodium Azide. 1000mL, Sterile.

Quantity

Minimum is set to 4 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

1X PBS-TBN Buffer is a phosphate-buffered saline containing Tween-20, bovine serum albumin, and sodium azide. It provides a balanced salt environment suitable for routine laboratory workflows involving proteins and antibodies.

This buffer is used for washing and blocking steps in immunoassays and immunofluorescence, and as an antibody diluent. Sodium azide functions as a preservative for short-term storage of aqueous antibody and protein solutions.

  • 150mM Sodium Chloride
  • 50mM Monosodium Phosphate
  • 1.00% Bovine Serum Albumin
  • 0.10% Sodium Azide
  • 0.05% Tween-20 (v/v)
  • ELISA: Plate blocking, antibody/sample diluent, and wash buffer to reduce nonspecific binding and stabilize proteins.

  • Western Blotting: Membrane blocking and antibody incubation/wash buffer to minimize background during detection.

  • Immunohistochemistry (IHC)/Immunocytochemistry (ICC)/Immunofluorescence (IF): Blocking and antibody diluent/wash buffer for fixed tissues and cells.

  • Flow Cytometry: Antibody staining buffer and wash for fixed cells to reduce nonspecific interactions and preserve reagents.

  • Bead-Based Immunoassays (e.g., Luminex): Sample and antibody diluent, blocking, and wash buffer to improve assay specificity.

  • Antibody Dilution/Storage: Short-term stabilization and dilution of antibodies and protein reagents with preservative.

  • Plate Washing: Low-background wash buffer for high-throughput immunoassays and microplate workflows.

  • General Laboratory Washes: Gentle surfactant-containing PBS for rinsing fixed cells, tissues, and surfaces in immunoassay workflows.

  • pH: 7.40 ± 0.10
Storage Temperature
Refrigerated
Shelf Life
730 days
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