Genomic DNA Extraction: SDS/EDTA lysis buffer for efficient isolation of high-molecular-weight DNA from cells and tissues.

Bacterial Cell Lysis: Disrupts cell walls and membranes to release nucleic acids from Escherichia coli, Staphylococcus aureus, and other bacteria.

Nuclease Inactivation: EDTA chelates divalent cations and SDS denatures proteins to inhibit DNases/RNases during nucleic acid purification.

Pre-Extraction Sample Preparation: Detergent-based lysis prior to phenol–chloroform or column-based DNA cleanup.

Protein Denaturation: SDS-driven unfolding and solubilization of proteins to reduce contaminants in DNA preparations.

Membrane Solubilization: Disrupts cellular and organelle membranes to improve nucleic acid yield from tough samples.

Southern Blot and Restriction Digest Prep: Generates clean, high-quality DNA suitable for downstream enzymatic workflows.

Microbial Genomics Workflows: Lysis and DNA prep from Gram-negative and Gram-positive organisms, including Escherichia coli and Bacillus subtilis.