C. elegans Culture and Maintenance: Routine propagation of C. elegans on lawns of Escherichia coli OP50 supported by 0.30% NaCl, 0.25% Tryptone, 2.00% Agar, and 5 µg/mL cholesterol.

Genetic Crosses and Strain Propagation: Setting up crosses, maintaining mutants, and isolating lines on standardized 60 mm plates.

RNAi by Feeding: Knockdown studies using Escherichia coli HT115(DE3) expressing dsRNA on buffered plates (25 mM potassium phosphate; 1 mM CaCl2, 1 mM MgSO4).

Developmental and Life-History Assays: Synchronization, growth rate, lifespan, and fecundity measurements under defined nutritional conditions.

Behavioral Assays: Chemotaxis, locomotion, egg-laying, and feeding (pharyngeal pumping) on reproducible agar surfaces.

Drug and Toxicology Screening: Plate-based exposure and dose–response testing for small molecules and environmental toxins.

Host–Pathogen Interaction Studies: Infection assays with Pseudomonas aeruginosa PA14, Serratia marcescens, or Enterococcus faecalis to assess innate immunity.

Transgenics and Reporter Analysis: Screening and fluorescence imaging of plate-grown animals for expression and phenotypes.

Dauer and Stress Response Studies: Induction and analysis of dauer formation and stress tolerance under controlled plate conditions.

Dietary and Microbiome Experiments: Testing defined bacterial diets and microbial communities (e.g., non-OP50 Escherichia coli strains) for nutritional effects.