Primary Isolation and Cultivation: Solid medium for recovery of Mycobacterium tuberculosis complex and other mycobacteria from decontaminated clinical specimens.

Colony Enumeration (CFU): Quantification of viable mycobacteria in cultures or infection models.

Antimicrobial Susceptibility Testing: Proportion/MIC testing of drugs against Mycobacterium spp. on solid medium.

Subculture and Maintenance: Routine propagation and short-term storage of mycobacterial isolates.

Colony Morphology Assessment: Evaluation of growth rate, texture, and pigmentation of Mycobacterium colonies.

Selective Plating with Additives: Use with antibiotics or supplements to select for mutants or recombinant strains.

Non-tuberculous Mycobacteria Studies: Cultivation of species such as Mycobacterium avium complex, Mycobacterium kansasii, and Mycobacterium abscessus.

Clinical Laboratory Workflows: Parallel plating with 7H11 or LJ for comprehensive mycobacterial recovery.