Routine Cultivation and Maintenance: Supports growth of non-fastidious bacteria such as Escherichia coli for everyday culture work.

Colony Isolation: Used for streaking and obtaining single colonies from mixed or starter cultures.

Antibiotic Selection: Enables selection of plasmid-bearing transformants by incorporating antibiotics into the medium.

Blue-White Screening: Facilitates α-complementation using X-gal/IPTG for recombinant clone identification.

CFU Enumeration: Employed for spread/spot plating to determine viable cell counts.

Genetic Manipulations: Supports selection after transformation, conjugation, or transduction in Escherichia coli and related bacteria.

Phage Work: Serves as a base layer for soft-agar overlay plaque assays with coliphages.

Strain Preservation: Used to prepare slants/plates for short-term storage and recovery of laboratory strains.