Propagation of E. coli strains: Routine growth in liquid culture and on agar plates for maintenance and experiments.

Plasmid cloning and amplification: Growth of transformants for minipreps/midipreps and downstream molecular biology.

Antibiotic selection (low-salt compatible): Selection with salt-sensitive antibiotics such as Zeocin/bleomycin.

Transformation outgrowth: Short recovery and expansion of transformed E. coli prior to plating or selection.

Colony isolation: Streaking and picking colonies from LB Luria agar plates.

Starter and overnight cultures: Inoculation of E. coli for cloning workflows and protein expression setups.

Glycerol stock preparation: Culturing prior to cryopreservation of E. coli strains.

General recombinant DNA workflows: Support medium for routine subcloning, screening, and verification steps.