Bacterial Culture and Colony Isolation: Growth and isolation of single colonies from mixed or pure cultures of non-fastidious bacteria such as E. coli and Bacillus subtilis.

Plasmid Transformation Selection: Selection of recombinant E. coli transformants when supplemented with appropriate antibiotics.

Colony-Forming Unit (CFU) Enumeration: Quantification of viable bacteria by serial dilution and plating.

Cloning and Screening: Blue/white screening and colony PCR workflows when supplemented with X-gal/IPTG and suitable vectors.

Strain Maintenance and Storage: Short-term storage and routine propagation of laboratory strains on plates.

Phage Work: Preparation of bacterial lawns (e.g., E. coli) for coliphage plaque assays using soft agar overlays.

Antibiotic Resistance Screening: Assessment of selectable markers or resistance phenotypes on supplemented plates.

Conjugation and Mating Assays: Selection of transconjugants in bacterial mating experiments (e.g., E. coli donor/recipient strains) with selective supplements.

Environmental Isolate Culturing: Initial culturing and colony picking from environmental or microbiome samples of non-fastidious bacteria.

Quality Control Checks: Monitoring for contamination of media, reagents, and workflows via surface streaking and plating.