Isolation of Single Colonies: Streaking to obtain clonal isolates of Escherichia coli and other nonfastidious bacteria.

Antibiotic Selection of Transformants: Plating transformed E. coli on LB agar supplemented with antibiotics to select plasmid-bearing colonies.

CFU Enumeration: Spread or pour plating for colony-forming unit counts from liquid cultures or environmental samples.

Maintenance of Bacterial Strains: Routine culture and short-term storage of lab strains (e.g., E. coli, Bacillus subtilis).

Cloning Workflows: Recovery and screening of colonies after ligation, Gibson assembly, or Golden Gate cloning.

Blue-White Screening: Screening of recombinant clones on LB agar with X-gal/IPTG and appropriate antibiotic.

High-Density Library Screening: Large-format plating (245 mm) for colony libraries and automated picking.

Replica Plating: Transferring colonies to selective/nonselective media to assess phenotypes or auxotrophies.

Phage Plaque Assays: Preparing bacterial lawns (e.g., E. coli) on LB agar with soft agar overlays for bacteriophage titration.

Conjugation and Mating Assays: Selection of transconjugants after bacterial conjugation on selective LB agar.