Routine Cultivation: Growth and maintenance of non-fastidious bacteria such as Escherichia coli, Bacillus subtilis, and Salmonella enterica.

Plasmid Propagation: Amplification of plasmid DNA in Escherichia coli for mini-, midi-, and maxi-preps.

Antibiotic Selection: Selection and maintenance of recombinant strains by supplementing with appropriate antibiotics.

Transformation Recovery: Post-heat shock or electroporation outgrowth to allow expression of antibiotic resistance markers.

Starter and Overnight Cultures: Inoculation of single colonies for downstream cloning, sequencing, or protein work.

Glycerol Stock Preparation: Culturing prior to mixing with glycerol for long-term cryopreservation of strains.

Phage and BAC Workflows: General-purpose host growth medium for workflows involving bacteriophage or large constructs in Escherichia coli.

General Microbial Workflows: Colony picking, inoculum expansion, and routine QC of lab strains.