Antibiotic Selection of Transformants: Selects colonies carrying kanamycin-resistance markers (e.g., nptII/neo) in Escherichia coli at 100 µg/mL.

Plasmid Maintenance: Supports stable propagation of kan-marked plasmids in Escherichia coli, Bacillus subtilis, and Agrobacterium tumefaciens.

Cloning/Transformation Screening: Enables screening of colonies after chemical transformation or electroporation on LB (1.0% tryptone, 0.5% yeast extract, 1.0% NaCl, 1.5% agar) with kanamycin.

Conjugation/Mating Selection: Selects kan-resistant transconjugants in biparental/triparental matings involving Escherichia coli and Agrobacterium tumefaciens.

Colony Isolation and Purification: Streaking to isolate single kan-resistant colonies and eliminate non-resistant contaminants.

CFU Enumeration of Transformants: Counts colony-forming units to quantify transformation or transfection efficiency under kan selection.

Recombinant Strain Propagation: Maintains kan-resistant expression strains prior to induction and downstream protein production.

Colony PCR and Sequencing Prep: Picking kan-selected colonies for rapid colony PCR and plasmid verification.

Chromosomal Knockout/Insertion Selection: Selects strains with kan-marked genomic edits (e.g., Keio-style knockouts) in Escherichia coli.

Glycerol Stock Starter Plates: Generates clean, single-colony sources for cryostocks of kan-resistant strains.