Antibiotic-Selective Cloning and Transformation: Selection of chloramphenicol-resistant transformants (34 µg/mL) in Escherichia coli following heat-shock or electroporation.

Plasmid Maintenance: Stable propagation of cat-marked plasmids in Escherichia coli and other chloramphenicol-resistant lab strains.

Colony Isolation and Streaking: Isolation of single colonies from mixed cultures under chloramphenicol selection for strain purification.

CFU Enumeration: Quantification of viable chloramphenicol-resistant cells after transformations, ligations, or library constructions.

Library Screening: Screening genomic or cDNA libraries carried on chloramphenicol-selectable vectors.

Strain Verification and Replica Plating: Verification of genotype/plasmid retention by replica plating onto chloramphenicol-selective LB agar.

Conjugation/Mating Selections: Recovery of transconjugants carrying chloramphenicol resistance markers on LB agar.

Protein Expression Workflows: Maintenance of expression strains bearing chloramphenicol-selectable plasmids prior to induction.

Stock Recovery and Passage: Revival of glycerol stocks and short-term culture maintenance of chloramphenicol-resistant Escherichia coli or Bacillus subtilis.

Microbial Quality Control: Monitoring contamination while preserving chloramphenicol-marked host strains such as Escherichia coli or Salmonella enterica.