Antibiotic Selection of Transformants: Selects for colonies carrying ampicillin-resistant plasmids after transformation of Escherichia coli.

Plasmid Maintenance on Solid Media: Maintains selection pressure to prevent plasmid loss during routine subculture of E. coli.

Colony Isolation and Purification: Streaks for single colonies to obtain clonal E. coli populations harboring Amp-resistant plasmids.

Colony Screening: Screens transformants by colony PCR or restriction analysis after picking Amp-resistant E. coli colonies.

Transformation Efficiency Testing: Quantifies CFUs to benchmark competent E. coli cell performance under Amp selection.

Cloning and Subcloning Workflows: Recovers recombinant plasmids post-ligation or Gibson assembly by selecting Amp-resistant E. coli clones.

Library Construction and Screening: Selects ampicillin-marked genomic, cDNA, or mutagenesis libraries in E. coli.

Conjugation and Mating Selections: Recovers transconjugants when the selectable marker confers ampicillin resistance in E. coli.

Expression Strain Recovery: Recovers and maintains expression plasmids with Amp resistance in E. coli prior to induction in liquid culture.

Quality Control of Plasmid Preparations: Verifies plasmid integrity and antibiotic marker function by re-plating on Amp with E. coli.