Selection of Ampicillin-Resistant Transformants: Isolates colonies carrying amp-resistance plasmids on 1.0% Tryptone, 0.5% Yeast Extract, 1.0% NaCl, 1.5% Agar with 10 µg/mL ampicillin.

Cloning and Screening: Supports colony PCR, restriction screening, and sequence verification of recombinant clones from E. coli.

Plasmid Maintenance and Propagation: Maintains selective pressure to preserve plasmids in E. coli cultures.

Streaking for Single Colonies: Enables isolation of pure ampicillin-resistant colonies for downstream workflows.

Blue-White Screening (with Supplements): Used with X-gal/IPTG for α-complementation-based screening of inserts in E. coli.

Transformation Efficiency and CFU Enumeration: Quantifies colony-forming units of ampicillin-resistant transformants.

Expression Screening: Selects plasmid-bearing E. coli for small-scale protein expression tests.

Plasmid Stability Assays: Evaluates retention of ampicillin-selectable plasmids over passages in E. coli.

Library Construction and Selection: Enriches correctly assembled clones after ligation, Gibson, or Golden Gate workflows.

Short-Term Strain Archiving: Stores and recovers plasmid-bearing E. coli colonies for subsequent culturing.