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I3326

IPTG. 100g.

Availability

Ships in 6 - 14 Days

Container Size

250cc

Item Count

1 Dry Container(s)

Price per Item

$704.00

Fill Volume

101 g

COA
COA

I3326

IPTG. 100g.

Quantity

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Description
Formulation
Common Applications
Storage

Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a non-metabolizable lactose analog used to induce transcription from lac operator–regulated promoters in bacteria. It binds the LacI repressor, facilitating expression from systems such as lac, tac, trc, and T7-lac promoters in E. coli and related hosts.

IPTG is commonly employed for controlled expression of recombinant proteins and in blue-white screening workflows in conjunction with chromogenic substrates. Its use enables regulated activation of operon-controlled genetic constructs without being consumed by cellular metabolism.

  • Isopropyl β-D-1-thiogalactopyranoside(IPTG)

  • Recombinant Protein Expression: Induces lac/tac/trc and T7-lac promoter systems for high-level protein production in Escherichia coli (e.g., BL21(DE3)).

  • Blue-White Colony Screening: Used with X-gal on selective plates to distinguish recombinant clones in E. coli.

  • T7 RNA Polymerase Induction: Triggers T7 RNAP expression in DE3 lysogens to drive pET/T7 promoter-based constructs in E. coli.

  • Reporter Gene Assays: Controlled induction of lac-regulated reporters (e.g., lacZ, GFP) to quantify promoter activity in E. coli.

  • Bacterial Two-Hybrid/Interaction Studies: Regulates expression of bait/prey fusions under lac control for interaction mapping in E. coli.

  • Gene Regulation and Functional Studies: Modulates lac-controlled genes for metabolic, complementation, or pathway analyses in E. coli.

  • Expression Optimization: Fine-tunes induction timing and concentration to improve protein folding/solubility in E. coli cultures (flasks and bioreactors).

  • Inducible Systems in Other Bacteria: Activates lacI/spac-based IPTG-inducible promoters in engineered Gram-positive hosts such as Bacillus subtilis.

Storage Temperature
Frozen
Shelf Life
days
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