Nucleic Acid Hybridization (Southern/Northern): Lowers DNA/RNA melting temperature to improve probe specificity and stringency in hybridization buffers.

In Situ Hybridization (ISH/FISH): Facilitates probe binding in tissues and cells for chromosomal and microbial detection with controlled stringency.

PCR Additive for GC-Rich Templates: Reduces secondary structure to enhance amplification efficiency and specificity in difficult amplicons.

Denaturing PAGE of RNA/DNA: Used with urea in denaturing gels and sample buffers to maintain single-stranded nucleic acids during electrophoresis.

RNA Handling and Storage: Maintains RNA in a denatured state and helps inhibit secondary structure formation during storage and processing.

Microarray Hybridization: Improves hybridization kinetics and specificity on DNA/RNA microarray platforms.

Sequencing and Capillary Electrophoresis: Acts as a denaturant to keep templates single-stranded for accurate sizing and base-calling.

Probe Preparation and Labeling: Aids in maintaining probes single-stranded during labeling and purification workflows.