Nucleic Acid Preservation (TE Buffer): Chelates Mg2+ to inhibit DNases/RNases; used to store and stabilize DNA/RNA.

Agarose Gel Electrophoresis Buffers (TAE/TBE): Provides EDTA to protect nucleic acids from nuclease activity during electrophoresis.

Quenching Mg2+-Dependent Reactions: Stops polymerase, ligase, and restriction enzyme reactions by sequestering divalent cations.

Protein Purification/Storage: Inhibits metalloproteases and chelates trace metals to prevent protein degradation and oxidation.

IMAC Resin Stripping/Regeneration: Removes Ni2+/Co2+ from charged affinity resins during cleanup or regeneration.

Cell Dissociation in Tissue Culture: Used in Ca2+/Mg2+-free buffers (e.g., PBS-EDTA) to gently detach adherent cells.

Competent Cell Preparation: Chelates divalent cations to permeabilize outer membranes in Escherichia coli workflows.

Biofilm and Membrane Studies: Disrupts Ca2+-dependent biofilms and increases outer membrane permeability in Pseudomonas aeruginosa.

DNA/RNA Sample Transport: Added to collection/stabilization buffers to preserve integrity of nucleic acids.

Tissue Decalcification (Histology): Slowly chelates calcium from mineralized tissues for morphology-preserving decalcification.