Yeast Auxotrophic Selection: Selects and maintains Saccharomyces cerevisiae transformants carrying LEU2 and URA3 markers in a defined medium with 2.0% glucose and 0.5% ammonium sulfate.

Dual-Plasmid Maintenance: Co-maintenance of LEU2/URA3 plasmids for cloning, expression, and stability studies in yeast.

Yeast Two-Hybrid and Interaction Assays: Supports assays requiring LEU2 and URA3 selection for bait/prey or reporter constructs.

CRISPR/Cas9 Genome Editing: Selection of edited yeast using LEU2/URA3 markers and workflows preceding URA3 counterselection (e.g., 5-FOA).

Transformation and Recovery: Outgrowth and enrichment of yeast transformants under -Leu -Ura selection.

Plasmid Shuffling and Retention/Loss Assays: Maintenance or eviction of LEU2/URA3-marked plasmids, including pre-5-FOA culture steps.

Growth and Phenotypic Assays in Defined Conditions: Controlled studies of growth, starvation responses, and gene expression with 2.0% glucose as carbon and 0.5% ammonium sulfate as nitrogen source.