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C3860

CM Galactose Agar Plates minus Tryptophan. 100mm, 20 Plates per Sleeve, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

100mm

Item Count

20 Plate(s)

Price per Item

$113.00

Fill Volume

25 mL

C3860

CM Galactose Agar Plates minus Tryptophan. 100mm, 20 Plates per Sleeve, Sterile.

Quantity

Minimum is set to 4 for this product*

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Description
Formulation
Common Applications
Storage

CM Galactose Agar Plates minus Tryptophan are a defined agar medium formulated without tryptophan, using galactose as the primary carbon source and ammonium sulfate as the inorganic nitrogen source. The medium supports growth of tryptophan-prototrophic yeast strains, enables selection of TRP1-based auxotrophic markers, and is suitable for experiments requiring galactose-inducible expression systems.

  • 2.0% D-(+)-Galactose
  • 0.5% Ammonium Sulfate
  • Galactose-Inducible Gene Expression: Induces GAL1/GAL10 promoter-driven expression using 2.0% D-(+)-Galactose as the carbon source.

  • TRP1-Based Plasmid Selection: Selects and maintains Saccharomyces cerevisiae transformants carrying TRP1 on tryptophan dropout plates.

  • Yeast Two-Hybrid Workflows: Supports bait/prey maintenance on -Trp selection in GAL-induced systems.

  • Auxotrophic Complementation Tests: Verifies TRP1 complementation and genotype confirmation in yeast strains.

  • Replica Plating and Colony Screening: Enables phenotypic screening on defined minimal medium with galactose.

  • CRISPR/Cas9 Editing Pipelines: Selects TRP1-marked Cas9/gRNA plasmids during genome editing in yeast.

  • Metabolic and Catabolite Repression Studies: Evaluates growth and gene regulation on galactose versus glucose.

  • Nitrogen Source-Dependent Assays: Utilizes 0.5% ammonium sulfate as a defined nitrogen source for reproducible growth kinetics.

Storage Temperature
Room Temperature
Shelf Life
300 days
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