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C2190

CTAB Extraction Solution. 250mL.

Availability

Ships in 6 - 14 Days

Container Size

250mL

Item Count

1 Bottle(s)

Price per Item

$73.00

Fill Volume

250 mL

C2190

CTAB Extraction Solution. 250mL.

Quantity

Minimum is set to 4 for this product*

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Description
Formulation
Common Applications
Storage

CTAB Extraction Solution is a ready-to-use buffer for cetyltrimethylammonium bromide–based isolation of nucleic acids from plant tissues, fungal samples, and selected bacteria (e.g., Agrobacterium spp.). It is prepared at pH 8.0 and provides ionic strength and chelation conditions commonly used to separate nucleic acids from polysaccharides and polyphenols.

For typical workflows, prewarm the solution and perform lysis at 60–65 °C, followed by extraction with chloroform:isoamyl alcohol (24:1) and alcohol precipitation of nucleic acids. RNase A may be added for RNA removal, and β-mercaptoethanol is commonly added immediately before use as required by the sample type.

  • 2.0% CTAB
  • 100mM Tris-HCl
  • pH 8.0
  • 20mM EDTA
  • pH 8.0
  • 1.4M Sodium Chloride
  • Plant Genomic DNA Extraction: Efficient lysis and purification of DNA from polysaccharide- and polyphenol-rich tissues (leaves, roots, seeds, woody plants).

  • Fungal and Yeast DNA Extraction: Isolation of high-quality genomic DNA from fungi and yeasts where polysaccharides interfere with downstream analyses.

  • Bacterial Genomic DNA Extraction: Recovery of genomic DNA from Gram-negative and Gram-positive bacteria (e.g., Escherichia coli, Bacillus subtilis).

  • Environmental/Soil Metagenomic DNA Isolation: Extraction of community DNA from soil, rhizosphere, and sediment samples with high humic substances.

  • Algal and Cyanobacterial DNA Extraction: Purification of nucleic acids from algae and cyanobacteria (e.g., Synechococcus) with robust cell walls and polysaccharides.

  • Removal of Polysaccharides and Polyphenols: CTAB-mediated separation of inhibitors to improve downstream PCR, qPCR, and sequencing.

  • High-Molecular-Weight DNA Preparation: Gentle extraction of long genomic DNA suitable for long-read sequencing and optical mapping.

  • Insect and Arthropod DNA Extraction: Isolation of genomic DNA from chitin- and pigment-rich specimens for barcoding and genomics.

  • Microbiome DNA Extraction from Plant Tissues: Co-extraction of host and associated microbial DNA from leaves, roots, and seeds with reduced inhibitor carryover.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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