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A4201

Antibiotic Medium #1. 500mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$87.00

Fill Volume

550 mL

COA
COA

A4201

Antibiotic Medium #1. 500mL, Sterile.

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Storage

Antibiotic Medium #1 is a solid medium specified in compendial antibiotic assay methods for the cylinder-plate (diffusion) technique. It is used as the seed-layer medium for designated test organisms in quantitative potency determinations, providing consistent growth and diffusion characteristics for zone-of-inhibition measurements as outlined in USP/EP/JP procedures.

Typical applications include assays employing Staphylococcus aureus and Bacillus subtilis strains recommended for specific antibiotics. The medium supports standardized test conditions for establishing assay validity, including defined inoculum densities, incubation parameters, and reference standard comparisons.

  • Antibiotic Medium #1

  • Microbiological Potency Assays: Used in compendial agar diffusion (cylinder-plate) assays to quantify antibiotic activity with Antibiotic Medium #1.

  • Seed Layer Preparation: Forms the inoculated seed agar layer for bioassays using organisms such as Bacillus subtilis, Micrococcus luteus, and Staphylococcus aureus.

  • Quality Control of Antibiotics: Employed by QC labs to verify potency of finished products, intermediates, and raw materials.

  • Standard Curve Generation: Supports preparation of reference standard dose–response curves for assay calibration.

  • Method Validation and Training: Utilized during validation, verification, and analyst training for microbiological assay workflows.

  • Assay of Beta-lactams and Other Classes: Suitable for compendial assays of penicillins, cephalosporins, and related antibiotics with Antibiotic Medium #1.

  • In-Process Testing: Applied to monitor potency during manufacturing and stability studies of antibiotic formulations.

Storage Temperature
Room Temperature
Shelf Life
730 days
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