Nucleic Acid Precipitation and Cleanup: Prepared from 7.5M Ammonium Acetate to 2–3 M final for ethanol/isopropanol precipitation of DNA/RNA, removing salts, phenol, and dNTPs.

LC–MS/HPLC Mobile Phase Buffer: Diluted to 1–100 mM as a volatile acetate buffer compatible with mass spectrometry for small molecules, metabolites, and nucleotides.

Oligonucleotide Desalting/Buffer Exchange: Used at 10–100 mM to replace nonvolatile salts prior to MS or lyophilization.

Protein Precipitation and Detergent/Salt Removal: Employed with cold methanol/acetone (e.g., 10–50 mM) to precipitate proteins and remove SDS, lipids, and salts for proteomics.

Capillary Electrophoresis: Serves as a volatile running buffer (5–50 mM) for separation of amino acids, peptides, and metabolites.

Ion-Exchange Chromatography (MS-Compatible): Used as a volatile salt for elution gradients when fractions are analyzed by MS.

DNA/RNA Elution from Gels: Applied at 0.3–1 M to elute nucleic acids from polyacrylamide or agarose during gel extraction workflows.

Sample pH/Ionic Strength Adjustment: Provides near-neutral, volatile buffering (10–50 mM) for enzymatic digests and reactions requiring MS compatibility.

MALDI/ESI-MS Sample Preparation: Minimizes nonvolatile adducts and improves spray/crystal quality using low-millimolar ammonium acetate.

Metabolomics and Lipidomics Workflows: Supplies a volatile counterion for adduct control and reproducible ionization in positive/negative modes.