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A0107

5mg/mL Adenine Hemisulfate Supplement. 50mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

60mL

Item Count

1 Bottle(s)

Price per Item

$52.00

Fill Volume

50 mL

A0107

5mg/mL Adenine Hemisulfate Supplement. 50mL, Sterile.

Quantity

Minimum is set to 25 for this product*

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Description
Formulation
Common Applications
Storage

Adenine Hemisulfate Supplement is an adenine source for culture media that supports the propagation and maintenance of adenine-auxotrophic yeast strains. It is suitable for supplementing defined or rich media to provide exogenous adenine for strains with impaired de novo purine biosynthesis, including ade mutants of Saccharomyces cerevisiae.

Applications include routine cultivation and genetic maintenance of adenine-requiring strains and mitigation of adenine deficiency phenotypes observed in auxotrophic backgrounds.

  • 5mg/mL Adenine Hemisulfate supplement
  • Yeast Auxotroph Support: Supplements synthetic complete (SC) and minimal (SD) media to support growth of adenine-requiring (ade−) Saccharomyces cerevisiae strains using 5mg/mL Adenine Hemisulfate supplement.

  • Colony Color Suppression: Reduces red pigment accumulation in ade1/ade2 mutant yeast colonies to improve visual screening and phenotype consistency.

  • Dropout Media Formulation: Added to SC dropout media to balance nucleotide pools and minimize background effects in selection experiments.

  • Yeast Two-Hybrid Assays: Included in base media to maintain healthy reporter strains and reduce false positives/negatives linked to adenine limitation.

  • Recombinant Protein Expression in Yeast: Ensures robust host growth for ADE-marked strains during expression and scale-up.

  • CRISPR/Cas9 Editing Workflows (Yeast): Improves post-transformation recovery and selection consistency in ade-marked strains.

  • Genetic Screens and Selections: Standard supplement in yeast genetics to standardize growth across ADE genotypes.

  • Fermentation and Preculture Preparation: Stabilizes growth during starter and production cultures of adenine-auxotrophic yeast.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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