We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

2W1230

Water, 20L Bag, DNase/RNase/Endotoxin Tested, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

20L

Item Count

1 Bag(s)

Price per Item

$1,519.00

Fill Volume

20000 mL

2W1230

Water, 20L Bag, DNase/RNase/Endotoxin Tested, Sterile.

Quantity

Minimum is set to 4 for this product*

scroll

Description
Formulation
Common Applications
Quality Specifications
Storage

Water that is tested for DNase, RNase, and endotoxin is used as a nuclease-free, low-endotoxin solvent for molecular biology and cell-based workflows where enzymatic or endotoxin contamination can interfere with experimental measurements.

Typical uses include dilution and storage of nucleic acids and proteins, preparation of buffers and reagents, reconstitution of lyophilized materials, preparation of negative controls, and rinsing of equipment and consumables to minimize introduction of DNase, RNase, or endotoxin derived from Gram-negative bacteria such as Escherichia coli.

Each lot is tested for absence of detectable DNase and RNase activity and for endotoxin within specified limits using standard assays such as nuclease activity assays and the Limulus Amebocyte Lysate (LAL) test.

  • Water
  • PCR/qPCR and RT-qPCR: Nuclease-free water for template and reagent dilution in DNA and RNA amplification workflows.

  • cDNA Synthesis and RT Reactions: RNase-free water for reverse transcription and RNA handling.

  • NGS Library Preparation: Used for adaptor dilution, cleanup steps, and resuspension of libraries.

  • Buffer and Reagent Preparation: Preparation of Tris, PBS, TE, and other molecular biology buffers.

  • Agarose Gel and Electrophoresis Buffers: Used to prepare agarose gels and TAE/TBE running buffers.

  • Cell Culture Reagent Preparation: Endotoxin-tested sterile water for making media, supplements, and rinses.

  • Microbial Culture Media: Preparation of LB, TB, and minimal media for organisms such as Escherichia coli.

  • ELISA and Immunoassays: Dilution of antibodies, standards, and wash solutions.

  • Blotting Workflows: Preparation of transfer and wash buffers for Western/Northern/Southern blots.

  • Sample Dilution and Controls: Creation of blanks, standards, and serial dilutions for analytical assays.

  • Endotoxin: ≤0.25EU/mL
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
1095 days

Product Documents

20 L Bag Diagram
1 MB
PDF
Shows related citations for this product
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button