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2T1220

DNA Suspension Buffer (10mM Tris, 0.1mM EDTA), pH 8.0 1000mL, RNase/DNase, PCR Grade, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$116.00

Fill Volume

1000 mL

2T1220

DNA Suspension Buffer (10mM Tris, 0.1mM EDTA), pH 8.0 1000mL, RNase/DNase, PCR Grade, Sterile.

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Storage

DNA Suspension Buffer, pH 8.0, is a Tris-based solution with EDTA for resuspension, dilution, and storage of purified DNA. The buffering capacity maintains a stable pH, and EDTA provides chelation of divalent metal ions that can influence nuclease activity.

RNase/DNase-free and PCR Grade specifications make it suitable for workflows requiring minimized nuclease contamination and compatibility with downstream enzymatic applications, including PCR, cloning, and sequencing.

  • 10mM Tris (Hydroxymethyl) Aminomethane
  • 0.1mM EDTA Disodium Dihydrate
  • DNA Resuspension and Elution: Reconstitutes DNA pellets and elutes DNA from purification columns using 10mM Tris (Hydroxymethyl) Aminomethane, 0.1mM EDTA Disodium Dihydrate, pH 8.0.

  • Long-Term DNA Storage: Maintains nucleic acid integrity; Tris buffers pH while EDTA chelates divalent cations to inhibit nuclease activity.

  • PCR/qPCR Template Dilution: Dilutes DNA templates for amplification while minimizing nuclease degradation; low EDTA helps preserve Mg2+ availability.

  • NGS Library Preparation: Dilutes and stores DNA libraries, adapters, and barcoded amplicons prior to sequencing.

  • Sanger Sequencing: Stores and dilutes plasmids or PCR amplicons before capillary electrophoresis.

  • Cloning and Transformation: Resuspends ligated plasmids and prepares DNA for transformation into Escherichia coli.

  • Post-Enzymatic Cleanup Resuspension: Provides a neutral, nuclease-free matrix for DNA after restriction digests, ligations, or phosphatase treatments.

  • DNA Standards and Controls: Prepares calibration curves and control materials for qPCR, ddPCR, and fragment analysis.

  • Shearing/Fragmentation Workflows: Resuspends DNA before and after acoustic or enzymatic fragmentation.

  • PCR Product Purification Elution: Elutes and stores cleaned amplicons for downstream cloning or sequencing.

Storage Temperature
Room Temperature
Shelf Life
1460 days
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