qPCR Sample Preparation: Facilitates Proteinase K–mediated lysis and nucleic acid release from swabs, saliva, and tissues; 1.11M Sodium Chloride and 1.11% Sarkosyl enhance cell disruption and nuclease inactivation for downstream qPCR.

DNA/RNA Extraction: Detergent/salt-assisted protein denaturation and membrane solubilization to improve yield and purity of nucleic acids prior to qPCR quantification.

Microbial Lysis for qPCR: Rapid lysis of bacteria such as Escherichia coli and Staphylococcus aureus to enable sensitive detection and quantification by qPCR.

Viral Inactivation and Stabilization: Sarkosyl-based disruption aids inactivation of enveloped viruses and stabilizes released nucleic acids for reliable qPCR readouts.

Nuclease Inactivation: High salt (1.11M NaCl) and detergent (1.11% Sarkosyl) conditions help denature RNases/DNases, protecting target nucleic acids.

Tissue Digestion: Supports Proteinase K digestion of protein-rich or viscous samples (e.g., sputum, tissue homogenates) to reduce inhibitors and improve qPCR performance.

Inhibitor Reduction: Protein and lipid solubilization decreases PCR inhibitors, improving amplification efficiency and Ct consistency.

Environmental and Food Testing: Lysis of microbial cells from water, surfaces, and food matrices to prepare samples for contamination assessment by qPCR.