Removal of Genomic DNA from RNA Samples (Pre-RT-qPCR): Supports DNase I activity (Mg2+/Ca2+/Tris-HCl) to eliminate DNA contamination before reverse transcription and qPCR.

On-Column DNase Treatment During RNA Purification: Used with spin-column RNA kits to digest residual DNA while maintaining RNA integrity for downstream qPCR.

In-Solution DNase Digestion of RNA Preparations: Facilitates efficient DNase I digestion in tube-based workflows to prevent false-positive qPCR amplification from contaminating DNA.

Cleanup Prior to RT-ddPCR and RNA-Seq Library Prep: Removes trace DNA to improve sensitivity and accuracy in RT-ddPCR and RNA-seq workflows.

Reagent and Master Mix Decontamination: Applied to digest unintended DNA contaminants in qPCR reagents and master mixes to reduce background amplification.

Host DNA Depletion in Microbial RNA Isolation: Digests contaminating genomic DNA (e.g., E. coli) in bacterial RNA preps to enhance specificity of RT-qPCR assays.

General DNase I Reaction Buffering: Provides optimal ionic conditions (Mg2+ and Ca2+ with Tris-HCl) for robust DNase I performance across standard DNA digestion applications.