pH Adjustment of Media and Buffers: Used to raise and precisely control pH in culture media, buffers, and process intermediates.

Chromatography Column Cleaning and Regeneration: Applied for stripping and sanitizing ion-exchange and other resins with high-pH washes.

Cleaning-In-Place (CIP) of Bioprocess Equipment: Employed to clean and sanitize tanks, lines, and manifolds in closed, sterile systems.

Plasmid DNA Preparation (Alkaline Lysis): Utilized to lyse E. coli cells during plasmid isolation workflows.

Affinity Resin Sanitization (e.g., Protein A): Used for periodic high-pH sanitization to reduce bioburden and fouling.

Membrane and Filtration System Cleaning: Applied to clean UF/DF cassettes, TFF systems, and filter housings between runs.

Endotoxin Reduction and Surface Depyrogenation: High-pH treatment helps inactivate and remove endotoxins from equipment surfaces.

Neutralization and pH Correction: Used to neutralize acidic process pools and waste streams before disposal or downstream processing.

General Decontamination: Employed for surface decontamination against laboratory bacteria such as Escherichia coli and Staphylococcus aureus.