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20931

YPD Broth, Animal-Free, 10Kg.

Availability

Ships in 6 - 14 Days

Container Size

3.5 Gallon

Item Count

1 Bucket(s)

Price per Item

$1,920.00

Fill Volume

10000 g

20931

YPD Broth, Animal-Free, 10Kg.

Quantity

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Description
Formulation
Common Applications
Storage

YPD Broth, Animal-Free is a nutrient-rich liquid medium for the cultivation and maintenance of yeasts, including Saccharomyces cerevisiae. It is formulated with plant-derived peptone (Soytone), yeast extract, and glucose to provide amino acids, vitamins, and a fermentable carbon source suitable for routine propagation and general microbiology workflows involving Saccharomycetales.

This animal-free composition facilitates laboratories seeking non-animal components while maintaining the familiar YPD profile for culture expansion, strain handling, and biomass preparation in research and process development settings.

  • 3.38% Soytone
  • Animal-Free
  • 1.00% Yeast Extract
  • 2.00% Glucose
  • Yeast Cultivation and Propagation: Rich, animal-free medium for robust growth of yeast strains for routine culturing and scale-up.

  • Fermentation and Biomass Production: Supports high-density aerobic batch and fed-batch fermentations for biomass and metabolite production.

  • Recombinant Protein Expression in Yeast: Provides nutrients for expression workflows using yeast hosts and plasmid-based systems.

  • Transformation Recovery: Used for post-transformation/electroporation recovery to maximize viability and colony formation.

  • Antibiotic Selection in Yeast: Compatible with drug-based selection (e.g., G418, hygromycin B) for selecting engineered yeast on rich media.

  • Starter/Seed Culture Preparation: Reliable medium for preparing inocula for bioreactors and downstream processes.

  • Yeast Genetics Workflows: Employed for mating, pre-sporulation growth, and maintenance before transfer to selective or defined media.

  • Viability and Revival of Yeast Stocks: Used to revive cryostocks and assess CFU and growth kinetics on plates or in liquid culture.

Storage Temperature
Room Temperature
Shelf Life
1825 days
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