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HEPES Buffer Solutions

Our HEPES buffer solutions are formulated for biological and biochemical workflows that require stable pH control near physiological conditions. HEPES is a zwitterionic buffering agent widely used in cell culture, protein assays, and molecular biology applications, where minimal interference with biological processes is essential.
HEPES Buffer Solutions
What are HEPES buffer solutions?

HEPES buffer solutions are commonly used in biological systems due to their effective buffering range of approximately pH 6.8-8.2, making them well-suited for maintaining near-physiological conditions. Unlike some other buffering agents, HEPES exhibits minimal temperature-dependent pH changes and low reactivity with metal ions, supporting stable experimental conditions. HEPES buffer solutions' compatibility with biological systems makes them a preferred buffer in applications requiring minimal cytotoxicity and reliable buffering performance.

Why choose Teknova’s HEPES buffer solutions?

We offer a wide selection of off-the-shelf RUO- and GMP-grade HEPES buffer solutions that are supplied as ready-to-use formulations, simplifying workflow setup and minimizing variability associated with manual buffer preparation. Each formulation is manufactured to precise specifications to ensure consistent buffering capacity and reliable performance.


Our HEPES buffer solutions are designed to integrate seamlessly into routine laboratory operations, from cell culture and analytical biochemistry to process development and quality control. All products are manufactured under rigorous quality control standards to ensure pH accuracy, purity, and lot-to-lot consistency. We can also manufacture custom HEPES buffer solutions to your exact specifications, with options including adjusted concentrations, modified pH values, and alternative container formats to meet specialized research or manufacturing requirements.

Explore related products

All our products are fully customizable, from formulation to format to fill volume. Our experts can help find the ideal solution for your application and efficiently manufacture your custom product.

Frequently asked questions

You can find answers here for our most commonly asked questions. If you don't find the information you are looking for, get in touch with our team. We're here to help.

HEPES buffer solutions are used to maintain stable pH in cell culture, biochemical assays, protein purification, and molecular biology workflows.

HEPES has an effective buffering range of approximately pH 6.8 to 8.2, making it suitable for near-physiological conditions. See our guide for optimal pH.

HEPES provides stable pH control with minimal temperature sensitivity and low biological interference, making it ideal for maintaining consistent conditions in cell culture systems.

Yes. All Teknova HEPES buffer solutions are supplied pre-formulated and ready to use, eliminating the need for in-house preparation. If you don't see the formulation or container format you are looking for in our catalog, we can also manufacture custom HEPES buffer solutions to your exact specifications. Learn more about our custom process.

HEPES buffer solutions should be stored according to product specifications, typically at room temperature. The storage instructions and expiration date for your specific product can be found on your product label.

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

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