Plasmid Selection in E. coli: Selects and maintains chloramphenicol-resistant clones at 30 µg/mL on LB agar with 1.69% animal-free soytone, 0.50% yeast extract, 1.00% NaCl, and 1.00% glucose.

Clone Screening and Isolation: Enables colony isolation after transformation of chloramphenicol-marked vectors in E. coli.

CFU Enumeration Under Selection: Quantifies transformation efficiency or library titers for chloramphenicol-resistant populations.

BAC/PAC/Cosmid Maintenance: Supports stable propagation of large constructs using chloramphenicol markers in E. coli.

Catabolite Repression During Recovery: 1% glucose helps minimize basal expression from inducible promoters (e.g., lac, ara) during clone recovery and screening.

Gram-Positive Vector Selection: Selects chloramphenicol-resistant strains of Bacillus subtilis and Staphylococcus aureus where LB is appropriate.

Conjugation and Transposon Workflows: Selects for transconjugants or insertions carrying chloramphenicol resistance.

Animal-Free Microbial Culture: Supports workflows avoiding animal-derived components for regulatory, vaccine-adjacent, or ethical requirements.

Strain Archiving and Picking: Colony picking for glycerol stocks and routine maintenance of chloramphenicol-marked strains.

Stability and Potency QC: Replica plating to assess plasmid retention and chloramphenicol activity over time.