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Y1204

20% Yeast Extract Solution. 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$140.00

Fill Volume

1000 mL

Y1204

20% Yeast Extract Solution. 1000mL, Sterile.

Quantity

Minimum is set to 8 for this product*

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Description
Formulation
Common Applications
Storage

20% Yeast Extract Solution is a sterile-filtered nutrient supplement for microbiological media preparation. It provides peptides, amino acids, vitamins, and growth factors derived from yeast to support the cultivation of diverse microorganisms, including E. coli, Bacillus subtilis, and Lactobacillus species.

It is commonly used to enrich broths and agar in applications such as cloning, microbial propagation, and recombinant protein workflows. The solution is suitable for aseptic addition to autoclaved media after cooling.

Store at 2–8 °C. Do not freeze. Mix gently before use. Intended for laboratory use.

  • 20% Yeast Extract Solution
  • Microbial Media Supplement: Added to LB, TB, 2xYT, SOC, and nutrient broths as a rich source of peptides, amino acids, and vitamins; 20% Yeast Extract Solution enables rapid microbial growth.

  • Bacterial Cultivation: Supports robust propagation of laboratory strains such as Escherichia coli, Bacillus subtilis, and Staphylococcus aureus by providing readily available nitrogen and growth factors; 20% Yeast Extract Solution simplifies media formulation.

  • Recombinant Protein Expression: Enhances biomass accumulation in high-density cultures of Escherichia coli for plasmid maintenance and protein production; 20% Yeast Extract Solution improves culture performance in shaker flasks and bioreactors.

  • Fermentation Processes: Used as a complex nitrogen and vitamin source to boost metabolite and biomass yields in microbial fermentations; 20% Yeast Extract Solution supports consistent lot-to-lot performance.

  • Phage Propagation: Provides nutrients for host growth during bacteriophage amplification and titering (e.g., lambda, T4) in Escherichia coli; 20% Yeast Extract Solution promotes reliable host readiness.

  • Antibiotic and Secondary Metabolite Screens: Supports growth of producers such as Streptomyces spp. in discovery and optimization workflows; 20% Yeast Extract Solution supplies complex nutrients for metabolite expression.

  • Transformation Recovery Media: Supplements SOB/SOC for post-transformation recovery of Escherichia coli, improving viability and outgrowth; 20% Yeast Extract Solution accelerates recovery.

  • Selective and Enrichment Broths: Incorporated into enrichment media for food, water, and environmental testing to resuscitate stressed bacteria; 20% Yeast Extract Solution increases recovery rates.

  • Probiotic Cultivation: Provides growth factors for lactic acid bacteria such as Lactobacillus and Bifidobacterium; 20% Yeast Extract Solution supports culture scale-up and maintenance.

  • Buffered Media Preparation: Combines with peptones and salts to formulate complex buffered broths and plates; 20% Yeast Extract Solution ensures consistent nutrient availability across batches.

Storage Temperature
Room Temperature
Shelf Life
730 days
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