Blue-White Screening in Cloning: 40mg/mL X-Gal in Dimethyl Formamide used with IPTG for lacZ α-complementation to distinguish recombinant colonies in Escherichia coli.

lacZ Reporter Gene Assays: Chromogenic substrate for β-galactosidase to quantify gene expression in bacteria, yeast, and mammalian cells.

Phage Plaque Screening: Visual identification of recombinant bacteriophage via blue/white plaque differentiation on indicator plates.

Colony and Filter-Lift Assays: Detection of β-galactosidase activity on membranes for rapid screening of transformants.

BAC/Fosmid/Plasmid Library Screening: High-throughput blue-white selection to identify inserts across large construct libraries.

Senescence-Associated β-Gal Staining: Histochemical detection of cellular senescence via β-gal activity at acidic pH.

Transgenic and Tissue Staining (in situ): Visualization of lacZ-tagged cells and tissues in developmental and anatomical studies.

Agar Plate Indicator Supplementation: Added to LB or selective agar for routine cloning screens in E. coli.

β-Galactosidase Expression QC: Verification of inducible expression systems and strain phenotypes expressing lacZ.

Educational and Training Labs: Demonstrations of gene regulation and enzyme activity using visible blue product formation.