X1201

X-Gal. 1g

Availability

Ships in 6 - 14 Days

Container Size

30cc

Item Count

1 Dry Container(s)

Price per Item

$75.00

Fill Volume

1.1

See label

COA

X1201

X-Gal. 1g

Quantity

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Description

Formulation

Common Applications

Storage

X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) is a chromogenic substrate for β-galactosidase used for blue/white screening of recombinant E. coli in lacZ α-complementation systems. Enzymatic hydrolysis by β-galactosidase produces an insoluble blue indigo dye, allowing visual differentiation of colonies on selective media.

In practice, X-Gal is incorporated into agar plates, commonly at 20–40 µg/mL, together with an inducer such as IPTG and an antibiotic appropriate for the plasmid backbone, to identify plasmid-bearing transformants in E. coli. It is also used in plaque assays and filter-lift assays where β-galactosidase activity indicates the presence of functional lacZ.

X-Gal
Ultra pure grade

Blue/White Colony Screening: Substrate for β-galactosidase to identify recombinant clones via α-complementation in Escherichia coli on IPTG/X-Gal plates.
Reporter Gene Assays: Chromogenic detection of lacZ expression in fixed cells and tissues for β-galactosidase activity mapping.
Phage and Viral Plaque Screening: Visual identification of recombinant plaques expressing lacZ (e.g., λ, M13) on X-Gal-containing overlays.
Yeast Two-Hybrid and Bacterial Two-Hybrid: lacZ reporter readout using X-Gal for interaction-dependent β-gal activity.
Library and BAC/Cosmid Screening: Blue/white selection of recombinant constructs maintained in Escherichia coli.
Microbiology Coliform Detection: Colorimetric indication of β-galactosidase-positive coliforms such as Escherichia coli in water and food testing.
Inducible Expression Monitoring: Plate-based verification of lac-inducible systems (IPTG/X-Gal) for clone screening and promoter characterization.

Storage Temperature

Frozen

Shelf Life

730 days

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X-Gal. 1g

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Raising the bar on quality

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