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T1455

TMN200 Solution, pH 8.0, Research Grade. 2L, Sterile

Availability

Ships in 6 - 14 Days

Container Size

2L

Item Count

1 Bottle(s)

Price per Item

$86.00

Fill Volume

2000 mL

T1455

TMN200 Solution, pH 8.0, Research Grade. 2L, Sterile

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

TMN200 Solution, pH 8.0, Research Grade is a Tris-buffered saline with magnesium formulated to maintain stable pH and ionic strength in molecular biology workflows.

Suitable for routine preparation and dilution of nucleic acids and proteins, stabilization of enzyme-compatible conditions requiring magnesium, and for washing or resuspension steps in workflows involving laboratory strains such as E. coli.

  • 200mM Sodium Chloride
  • 1mM Magnesium Chloride
  • 20mM Tris
  • Bacteriophage Dilution and Storage: Maintains phage stability and infectivity during dilution series and short- to mid-term storage, commonly with Escherichia coli hosts.

  • Plaque Assays and Phage Adsorption: Supports efficient adsorption and plaque formation in soft agar overlays for coliphages on Escherichia coli.

  • Phage Lysate Preparation and Resuspension: Used to resuspend PEG/NaCl-precipitated phage pellets and clarify lysates while preserving particle integrity.

  • Elution of Phage from Samples: Facilitates recovery of phage from environmental or clinical matrices and membrane filters for downstream titering.

  • Host Cell Preparation for Infection: Serves as a wash or equilibration buffer for bacterial hosts (e.g., Escherichia coli) prior to phage infection.

  • Phage DNA Extraction Workflows: Provides a compatible ionic and pH environment for initial phage handling before nucleic acid purification.

  • pH: 8.00 ± 0.04
Storage Temperature
Room Temperature
Shelf Life
1095 days
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