SDS-PAGE Stacking Gel Preparation: Provides the 6.8 pH buffering required for the stacking gel to concentrate proteins before separation.

Laemmli Sample Buffer (Loading Dye) Preparation: Supplies the Tris-HCl component at pH 6.8 for preparing protein denaturation/loading buffers.

Protein Extraction and Lysis Buffers: Used to formulate lysis and extraction buffers where a stable pH 6.8 is desired to preserve protein integrity.

Enzyme Assays: Maintains a consistent pH 6.8 environment for enzymes with optimal activity near mildly acidic to neutral conditions.

Dialysis and Buffer Exchange: Provides controlled pH 6.8 conditions during desalting, dialysis, and buffer exchange of protein samples.

pH Adjustment of Laboratory Buffers and Media: Used to titrate and set solutions to pH 6.8 with a strong, well-defined buffering capacity.